Semi-Preparative HPLC Chromatography Ultra High Performance Liquid Chromatograph System Analytical Workstation

• After-sales Service: Lifetime Technical Support
• Warranty: 1 Year for Semi-Preparative HPLC Chromatography
• Type: Semi-Preparative HPLC Chromatography
• Load Way: Electronic Load
• Loading Method: Static Load
• Display: Digital
• Model NO.: HPLC
• Control: Computer Control
• Weight: 50kg
• Power Source: AC220V
• Product Name: Semi-Preparative HPLC Chromatography
• Flow Range: 0.01-100ml/Min
• Flow Accuracy: <1%
• Flow Stability: Rsd<0.3%
• Max Working Pressure: 30MPa
• Power Supply: AC220V 10%, 50Hz/100W
• Wavelength Range: 190-700nm
• External Dimension: 420*300*175mm
• Transport Package: Wooden Case
• Specification: ask the salesman
• Trademark: GOLD
• Origin: Chongqing. China

Product Description

Semi-preparative Hplc Chromatography Ultra High Performance
Liquid Chromatograph System Analytical Workstation


Semi-preparative/preparative HPLC is an analytical instrument used to separate and purify compounds. Semi-preparative/preparative HPLC offers greater sample handling and separation capabilities than conventional analytical HPLC.

Types of Chromatography
 

1. Partition chromatography
2. Norma-phase chromatography
3. Dispacement chromatography
4. Reversed-phase chromatography (RPC)
5. Size-excusion chromatography
6. Ion-exchange chromatography
7. Bioaffinity chromatography
8. Aqueous norma-phase chromatography

Applications of Chromatography

Data Workstation

How to Read a Chromatogram

A chromatogram refers to a graphical representation obtained through chromatography. An example of a chromatogram is shown in Figure below. It is a two-dimensional plot where the vertical axis represents the concentration measured by the detector's signal intensity, and the horizontal axis represents the time of analysis. When no substances are eluted from the column, a line parallel to the horizontal axis, known as the baseline, is plotted. The detector's response is based on the concentration of the desired compound in the elution band. The resulting plot typically exhibits a bell-shaped curve rather than a triangular shape, which is known as a "peak."

Retention time (tR) refers to the time interval between the point of sample injection and the highest point (apex) of the peak. The dead time (t0) is the time required for non-retained compounds (compounds that do not interact with the stationary phase) to travel from the injector to the detector.

Peak height (h) represents the vertical distance between the baseline and the apex of a peak, while the peak area (A), depicted in light blue, corresponds to the enclosed area between the peak and the baseline. These results are utilized for both qualitative and quantitative analyses of the components present in a sample.