Laboratory High Performance Liquid Chromatography System HPLC Machine
The high reliability and practicality of the liquid chromatography system ensure the long-term stable operation of the system,
which can be used for any quality control and routine analysis work. The computerized counter control workstation makes the experimental process simpler, and at the same time provides strong support for daily analysis work in various fields such as medicine, environmental protection, university scientific research, chemical industry, and food.
Technical indicators
| Parameter |
Range |
| Flow range |
0.001-10ml/min |
| Accuracy |
±1% |
| Precision |
0.25% |
| Pressure range |
0-42MPa |
| Gradient |
Binary |
| Gradient mode |
Gradient elution is self-controlling with internal software and can be controlled via PC and pumps |
| Power supply |
100 to 240 VAC, 50 to 60Hz,
80 watts |
| Dimension |
40cm(L)*30cm(W)*18cm(H) |
| Weight |
8.0kg |
| Lamp Source |
Tungsten Lamp |
| Light Path Design |
Monochromator with dual-path silicon photodiode detector |
| Wavelength Range |
190-700nm in 1nm increments, can be set from keyboard or RS232/485/USB |
| Bandwidth |
<6nm |
| Wavelength Accuracy |
±1nm |
| Wavelength Repeatability |
0.2nm |
| Absorption range |
0.0005AU/mV-4AU/mV |
| Data System Output |
4AU/V feed signal control, auto zero, supports dual output of integrator and recorder |
| Noise |
±0.5x10-5AU |
| Drift |
2x10-4AU (P-P) |
| Cuvette |
Standard Cone Cell (10mm range, 4.5ul volume), Semi-Preparative (5mm range, 3.5ul volume) |
Performance Characteristics
- High performance liquid chromatograph adopts static motor control circuit to realize the automatic feedback tracking function of motor control signal, which effectively improves the motor control precision and ensures the reliability and stability of the pump.
- Pump form: series or parallel double plunger.
- Floating self-correcting sealing ring technology and pump head self-cleaning function patented technology, which can extend the service life of the sealing ring.
- Convenient and easy-to-use flow and pressure correction functions ensure high accuracy and stability of the instrument.
- Designed with two gradient running programs: linear gradient and step gradient selectable.
- Programmed solvent class and compression factor for automatic compensation.
- Complete pre-cleaning function, more for rapid solvent replacement.
- Adoption of holographic dual grating technology.
- Apply high precision photodiode design with extended linear range.
- Convenient and unique light intensity gain factor self-correction function.
- Unique holmium glass cell design, full wavelength scanning, convenient and accurate wavelength correction.
- Universal cuvette design, easy to change between micro and macro cuvettes.
- Lamps are easy and quick to replace and can be used without calibration.
More Details
Working Principle
(a). In HPLC, the pump is responsible for delivering the mobile phase at a controlled flow rate.
(b). However, air bubbles can easily dissolve in the mobile phase under standard atmospheric pressure, which can cause issues like flow rate fluctuations and baseline noise/drift. To prevent this, a degassing unit is used to remove air bubbles from the mobile phase .
(c). Once the air bubbles have been removed, the mobile phase is delivered to the column. The sample injector then introduces a standard or sample solution into the mobile phase.
(d). To ensure consistent separation of compounds in the column, a column oven is used to maintain a constant temperature.
(e). As compounds elute from the column, they are detected by a detector placed downstream of the column.
(f). The detector signal is processed by a workstation to obtain a chromatogram, which is used to identify and quantify the individual compounds.
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