High Precision High Performance Liquid Chromatograph
Used in Pharmaceutical Industry&Chemical Analysis
HPLC Separation
HPLC achieves the separation and detection of individual compounds by exploiting the discrepancy in their movement speed through a column. The technique involves two distinct phases: the mobile phase, a liquid that dissolves the target compound, and the stationary phase, which interacts with the target compound within the column.
Within the column, the rate at which a component travels is determined by its level of interaction, such as through van der Waals forces, with the mobile and stationary phases. A higher affinity between a component and the mobile phase results in faster movement through the column alongside the mobile phase. Conversely, a stronger affinity with the stationary phase leads to slower movement. For instance, in Figure 3, the yellow component, which strongly associates with the mobile phase, swiftly traverses the column, while the pink component, with a strong affinity for the stationary phase, progresses slowly. The speed at which a compound elutes from the column is contingent upon its affinity with the stationary phase. Main Configurations